Bismark report for: /Volumes/Bay3/Software/bismark_v0.6.4/filtered_Unlabeled_NoIndex_L003_R1wID_trimmed.fastq Option '--directional' specified: alignments to complementary strands will be ignored Bowtie was run against the bisulfite genome of /Volumes/Bay3/Software/bismark_v0.6.4/genomes/ with the specified options: -q -k 2 --best --chunkmbs 512 Final Alignment report ====================== Sequences analysed in total: 139660651 Number of alignments with a unique best hit from the different alignments: 7205800 Mapping efficiency: 5.2% Sequences with no alignments under any condition: 126455782 Sequences did not map uniquely: 3586720 Sequences which were discarded because genomic sequence could not be extracted: 13816 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 3539113 ((converted) top strand) CT/GA: 3666687 ((converted) bottom strand) GA/CT: 0 (complementary to (converted) top strand) GA/GA: 0 (complementary to (converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 2412349 Final Cytosine Methylation Report ================================= Total number of C's analysed: 43070400 Total methylated C's in CpG context: 5173742 Total methylated C's in CHG context: 260101 Total methylated C's in CHH context: 1060970 Total C to T conversions in CpG context: 2571648 Total C to T conversions in CHG context: 7050506 Total C to T conversions in CHH context: 26953433 C methylated in CpG context: 66.8% C methylated in CHG context: 3.6% C methylated in CHH context: 3.8%